Table 2 Tomato transformation, selection on kanamycin supplemented medium and regeneration of transgenic plants
| Â | Istselection | IIndselection | |||||||
|---|---|---|---|---|---|---|---|---|---|
Experiment | No. of explants used (A ) | No. of responding explants (B) | % Response (B/A) | No. of shoot buds produced | No. of explants inoculated (C) | No. of responding explants (D) | % Response (D/C) | Antibiotic resistant plants produced (E) | % Frequency of transformation (E/A) |
1. | 138 | 50 | 36.23 | 63 | 186 | 83 | 44.62 | 39 | 28.20 |
2. | 104 | 49 | 47.11 | 60 | 65 | 39 | 60.00 | 22 | 21.15 |
3. | 123 | 59 | 47.96 | 76 | 226 | 76 | 33.62 | 28 | 22.76 |
4. | 194 | 124 | 63.91 | 103 | 319 | 142 | 44.51 | 54 | 27.83 |
| Â | 559c | 282c | 48.80d | 302c | 796c | 340c | 45.68d | 143c | 24.98d |
+Controla | 40 | 40 | 100 | 114 | 230 | 221 | 96.08 | - | - |
-Controlb | 52 | 8 | 15.3 | 0 | - | - | - | - | - |