Figure 4

The samples were embedded in paraffin. From paraffin blocks sections of 4 μm in width were taken and stained with hematoxylin-eosin, and were examined under light microscope (HE × 20). A: Melatonin group: The callus formation consisted of mostly well-developed trabecular bone (Black arrow). Haversian canals were seen in the section. Chondroid tissue areas were observed limitedly in the callus at the end of 82 days. (HE,×20). B: CAPE group: Callus was formed by predominantly trabecullar bone(Black arrow), and chondroid tissues(red arrow) were rarely observed in the callus at the end of 82 days. (HE,×20). C: Control group: Callus formation consisting of equal amounts of trabecular bone and chondroid tissue in the control(Black arrow) at the end of 82 days. (HE, ×20).