Table 1 Yield, purity, and integrity of total RNA isolated from frozen blood using three different extraction kits: TRI reagent, PAXgene, and NucleoSpin (n = 15)
From: Comparison of three different kits for extraction of high-quality RNA from frozen blood
RNA yield (μg/ml) | RNA purity (A260/A280) (A260/A230) | RNA integrity (RIN) | |
|---|---|---|---|
TRI reagent | 15.6 ± 8.7 (6.1-37.6) | 1.7 ± 0.1 (1.6-1.7) 1.4 ± 0.4 (0.7-2.1) | 3.2 ± 0.8 (1.6-5.0) |
PAXgene | 3.1 ± 1.7 (1.1-6.9) | 2.0 ± 0.1 (1.9-2.1) 1.1 ± 0.3 (0.6-1.5) | 6.0 ± 1.1 (4.0-7.7) |
NucleoSpin | 9.0 ± 5.5 (3.6-23.7) | 2.0 ± 0.0 (2.0-2.1) 1.8 ± 0.2 (1.5-2.1) | 6.4 ± 0.9 (4.6-7.6) |