Figure 5From: Effect on β-galactosidase synthesis and burden on growth of osmotic stress in Escherichia coliEffect of NaCl concentration on GFP synthesis and activity. (a) Normalized GFP fluorescence values measured in WT cells grown in a glycerol M9 medium containing various salt concentrations and saturating amount of IPTG (200 μmol l−1). Triangle (▲) and solid line respectively, represents experiment points and Hill equation fit.GFP expression is steeply inhibited with a Hill coefficient of 4.2 and half saturation constant of 0.4 mol l−1NaCl. (b) Normalized GFP fluorescence values measured in WT cells grown in a glycerol containing M9 medium in absence of and later exposed to 0.2 and 1.5 mol l−1 salt concentrations. It can be noted that fluorescence intensity was not affected. Diamond (♦) and square (■) respectively, represents the GFP activity after exposure to 0.2 and 1.5 M NaCl.Back to article page