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Figure 5 | SpringerPlus

Figure 5

From: Effect on β-galactosidase synthesis and burden on growth of osmotic stress in Escherichia coli

Figure 5

Effect of NaCl concentration on GFP synthesis and activity. (a) Normalized GFP fluorescence values measured in WT cells grown in a glycerol M9 medium containing various salt concentrations and saturating amount of IPTG (200 μmol l−1). Triangle (▲) and solid line respectively, represents experiment points and Hill equation fit.GFP expression is steeply inhibited with a Hill coefficient of 4.2 and half saturation constant of 0.4 mol l−1NaCl. (b) Normalized GFP fluorescence values measured in WT cells grown in a glycerol containing M9 medium in absence of and later exposed to 0.2 and 1.5 mol l−1 salt concentrations. It can be noted that fluorescence intensity was not affected. Diamond (♦) and square (■) respectively, represents the GFP activity after exposure to 0.2 and 1.5 M NaCl.

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