Figure 1
From: Effect on β-galactosidase synthesis and burden on growth of osmotic stress in Escherichia coli
Effect of NaCl concentration on the growth of E.coli at a fixed glycerol concentration of 1 g/L with and without 200 μM of IPTG: a. Specific growth rate at various NaCl concentrations. Filled and open Square, respectively, represent the growth rate at various salt concentrations with and without IPTG in the medium. Solid and dashed line represents Hill equation fit for growth rate with and without IPTG in the medium. The specific growth rate was normalized by μmax = h−1 for growth of WT on glycerol medium lacking salt. b. β-galactosidase enzyme activity at various salt concentrations for growth on medium with IPTG. The β-galactosidase activity was normalized by the maximum observed in the WT strain in a medium lacking salt. c. Burden, the relative reduction in growth rate or ф at various NaCl concentrations with and without saturating amount of IPTG in the medium. Filled and open Square, respectively, represent the ф at various salt concentrations with and without IPTG in the medium.