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Table 2 Full factorial design and experimental results of exo-PG activity in the second screening step of A. sojae ATCC 20235

From: Efficient polygalacturonase production from agricultural and agro-industrial residues by solid-state culture of Aspergillus sojae under optimized conditions

Trial	Experimental factors				Response
Trial	Inducer substrate	Inducer concentration (%)	HCl concentration (mM)	Time (d)	exo-PG activity (U/g)
1	Apple pomace	10	0.1	3.5	28.8
2	Sugar beet pulp	10	0.1	3.5	68.2
3	Apple pomace	40	0.1	3.5	57.5
4	Sugar beet pulp	40	0.1	3.5	104.2
5	Apple pomace	10	0.22	3.5	209.5
6	Sugar beet pulp	10	0.22	3.5	300.4
7	Apple pomace	40	0.22	3.5	165.5
8	Sugar beet pulp	40	0.22	3.5	-
9	Apple pomace	10	0.1	5.5	4.3
10	Sugar beet pulp	10	0.1	5.5	12.5
11	Apple pomace	40	0.1	5.5	38.1
12	Sugar beet pulp	40	0.1	5.5	88.8
13	Apple pomace	10	0.22	5.5	91.7
14	Sugar beet pulp	10	0.22	5.5	374.5
15	Apple pomace	40	0.22	5.5	225.2
16	Sugar beet pulp	40	0.22	5.5	-
17 – 18*	Apple pomace	25	0.16	4.5	118.2 ± 5.4
19 – 20*	Sugar beet pulp	25	0.16	4.5	277.5 ± 1.7

*The standard variation between the center point repetitions was below 5%.

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