Table 1 D-optimal design and experimental results of exo-PG activity in the first screening step of A. sojae ATCC 20235
Experimental factors | Response | |||||
|---|---|---|---|---|---|---|
Inducer substrate | Temperature (°C) | Inoculum (Total spores) | Time (d) | HCl concentration (mM) | exo-PG activity (U/g) | |
1 | Orange peel | 32 | 3 × 107 | 3 | 50 | 30.3 |
2 | Orange peel | 32 | 3 × 107 | 5 | 50 | 21.5 |
3 | Orange peel | 24 | 104 | 3 | 300 | - |
4 | Orange peel | 24 | 104 | 5 | 300 | - |
5 | Apple pomace | 24 | 104 | 3 | 50 | 20.0 |
6 | Apple pomace | 32 | 104 | 3 | 50 | 20.1 |
7 | Apple pomace | 24 | 3 × 107 | 5 | 300 | 216.3 |
8 | Apple pomace | 32 | 3 × 107 | 5 | 300 | 223.8 |
9 | Sugar beet pulp | 24 | 3 × 107 | 3 | 50 | 58.5 |
10 | Sugar beet pulp | 32 | 104 | 5 | 50 | 94.9 |
11 | Sugar beet pulp | 24 | 3 × 107 | 3 | 300 | - |
12 | Sugar beet pulp | 32 | 104 | 5 | 300 | - |
13 | Sugar beet with molasses | 24 | 104 | 5 | 50 | 76.7 |
14 | Sugar beet with molasses | 24 | 3 × 107 | 5 | 50 | 30.5 |
15 | Sugar beet with molasses | 32 | 104 | 3 | 300 | - |
16 | Sugar beet with molasses | 32 | 3 × 107 | 3 | 300 | - |
17 – 18* | Orange peel | 28 | 1,5 × 107 | 4 | 175 | 140.3 ± 3.8 |
19 – 20* | Apple pomace | 28 | 1,5 × 107 | 4 | 175 | 243.0 ± 10.3 |
21 – 22* | Sugar beet pulp | 28 | 1,5 × 107 | 4 | 175 | 407.5 ± 13.9 |
23 – 24* | Sugar beet with molasses | 28 | 1,5 × 107 | 4 | 175 | 291.2 ± 1.7 |