Figure 1

Co-transformation of Phlebia sp. strain MG-60 with p PbGPD -MG mnp 2 and p PbGPD-HPT . A, Detection of the MGmnp2 gene in 10 of 14 regenerated protoplasts co-transformed with pPbGPD-HPT and pPbGPD-MGmnp 2 by PCR amplification using primers PbGPD-prom-F1 and g MGmnp2-Asc- R1. M indicates a 1 kb ladder size marker, Wt indicates the wild type, HPT indicates the hygromycin resistant transformant, and M1–M14 indicate MGmnp2 regenerated transformants. B, MnP activity in Kirk’s high-nitrogen culture medium under aerobic condition for 3 d (white column) and 6 d (black column). One unit of MnP activity was defined as 1 μmol reaction product formed per minute. C, MnP activity of various strains in the extracts of Quercus wood powder medium under aerobic condition for 20 d. The tests, using three flasks per strain, were carried out independently. Data are means ± SE (n = 3) and values without a common superscript letter are significantly different at p <0.05.