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Table 1 Plackett burman design for initial screening of factors

From: Statistical design and optimization of single cell oil production from sugarcane bagasse hydrolysate by an oleaginous yeast Rhodotorula sp. IIP-33 using response surface methodology

Run order

Std. order

A

B

C

D

E

F

G

H

I

Response variable: Lipid wt.

Xylose

(NH4)2SO4

Na2HPO4

KH2PO4

Yeast extract

MgSO4

Time

pH

Temp

Experimental

Predicted

g

g

g

g

g

g

h

-

°C

g

g

1

7

+1

-1

-1

-1

+1

-1

+1

+1

-1

1.4363

1.4482

2

8

+1

+1

-1

-1

-1

+1

-1

+1

+1

1.0892

1.089

3

9

+1

+1

+1

-1

-1

-1

+1

-1

+1

0.7101

0.710

5

10

-1

+1

+1

+1

-1

-1

-1

+1

-1

1.3764

1.3883

6

6

-1

-1

-1

+1

-1

+1

+1

-1

+1

1.7299

1.7418

7

1

+1

+1

-1

+1

+1

+1

-1

-1

-1

0.5932

0.5932

8

5

-1

-1

+1

-1

+1

+1

-1

+1

+1

1.0931

1.0931

9

4

-1

+1

-1

+1

+1

-1

+1

+1

+1

1.4091

1.3972

10

3

+1

-1

+1

+1

-1

+1

+1

+1

-1

2.6138

2.6019

11

12

-1

-1

-1

-1

-1

-1

-1

-1

-1

0.5911

0.5792

12

11

+1

-1

+1

+1

+1

-1

-1

-1

+1

1.8509

1.8509