Figure 4

Effect of ATPase inhibitors resveratrol (RVT), piceatannol (PCT) or sodium azide on DNA gyrase reactivation in MC4100 stationary-phase cells. Cells were grown at 37°C in LB-MOPS medium. To induce the recovery of the DNA SC level, cell cultures were diluted 1:10 in pre-warmed LB-MOPS medium with or without the inhibitor. a: 1) Exponentially growing cells, 2) 48 hr stationary-phase cells, 3) stationary-phase cells diluted in LB-MOPS media and incubated 1 min, 4), 5) and 6) stationary-cells diluted in LB-MOPS with RVT 400 μM, 1.2 mM, or 2.0 mM, respectively. The diluted cultures were incubated 1 min. b: 1) Exponentially growing cells, 2) 48 hr stationary-phase cells, 3) stationary-phase cells diluted in LB-MOPS and incubated 1 min, 4), 5) and 6) stationary-phase cells diluted in LB-MOPS with PCT 100 μM, 200 μM or 300 μM, respectively. The diluted cultures were incubated 1 min. c: 1) Exponentially growing cells, 2) 48 hr stationary-phase cells, 3) stationary-phase cells diluted in LB-MOPS and incubated 10 min, 4) stationary-phase cells diluted in LB-MOPS-sodium azide 3 mM and incubated 10 min, 5) stationary-phase cells diluted in LB-MOPS-sodium azide 5 mM and incubated 5 min, 6) stationary-phase cells diluted in LB-MOPS-sodium azide 5 mM and incubated 10 min. Plasmid topoisomers were isolated and separated as described in Figure 1. Similar results were obtained in at least three independent experiments.