Figure 3

Effect of glucose as carbon source on DNA gyrase reactivation in MC4100 stationary-phase cells. Cells were grown in LB-MOPS at 37°C. To induce the recovery of the DNA SC level in stationary-phase cells, cell cultures were diluted 1:10 in pre-warmed LB-MOPS or 1:30 in a 0.86% NaCl solution with or without 0.4% glucose. 1) Exponentially growing cells; 2) 48 hr stationary-phase cells; 3) and 4) stationary-phase cells diluted in LB-MOPS and incubated for 3 or 5 min, respectively; 5) and 6) stationary-phase cells diluted in 0.86% NaCl and incubated for 3 or 5 min, respectively; 7) and 8) stationary-phase cells diluted in 0.86% NaCl-0.4% glucose and incubated for 3 or 5 min, respectively. Plasmid topoisomers were isolated and separated as described in Figure 1. Similar results were obtained in at least three independent experiments.