Figure 3

a-g Adventitious shoot regeneration of Adhatoda vasica . a Friable callus formation from petiole explants after 3 weeks of culture on MS medium supplemented with 0.25 mg l ⁻¹ 2,4-D and 0.25 mg l ⁻¹ TDZ. b Compact callus formation from petiole explants on MS medium supplemented with 0.25 mg l ⁻¹ NAA and 0.25 mg l ⁻¹ TDZ after 3 weeks of culture on the same medium. c Onset of organogenesis on MS medium supplemented with 0.25 mg l ⁻¹ NAA and 0.25 mg l ⁻¹ TDZ 4 weeks after culture initiation. d Adventitious multiple shoots on the same medium after 8 weeks of culture initiation. e Elongation growth of shoots on MS medium supplemented with 0.25 mg l ⁻¹ TDZ after 2 weeks of culture. f Rooting of in vitro shoot on MS medium supplemented with 0.5 mg l⁻¹ IBA after 4 weeks of culture. g Acclimatized plant after 8 weeks of transfer to garden soil. Bars =1 cm (a and b), 3 cm (c), 1 cm (d and e), 3 cm (f), and 1 cm (g).