Table 1 Extracellular laccase semi-purification from T. versicolor
Purification (Steps) | Volume (mL) | Total activity (U L-1) | Total protein Content (μg L-1) | Specific activity (U mg-1) | Purification (folds) |
|---|---|---|---|---|---|
Crude extract | 900 | 2700 | 2500 | 1 | 1 |
Precipitation (NH4)2SO4 | |||||
Sephacryl S-200 (1) | 60 | 8600 | 415 | 21 | 10 |
Precipitation (NH4)2SO4 | |||||
Sephacryl S-200 (2) | 44 | 10300 | 277 | 37 | 35 |