Figure 1From: Differentiation-inducing and anti-proliferative activities of lupeol on canine melanoma cellsUp-regulation of melanoma cell differentiation markers by lupeol. Four canine melanoma cells (2 × 105 cells) were treated without (white bar) or with 5 μM lupeol (gray bar) for 48 h, and the gene expression of MITF, TYR, and TRP-2 was measured by real-time RT-PCR. * p < 0.05, ** p < 0.01 vs untreated cells (n = 3).Back to article page