Bioremoval of the synthetic dye malachite green by marine Trichoderma sp

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Table 3 Analysis of variance table (ANOVA) for response surface methodology of main effects and interacting effects of parameters in quadratic model for the laccase production by Hypocrea lixii

Source	Sum of squares	df	Mean	F value	p-value Prob > F
Model	48.56277	14	3.468769	2.621997	0.0371*
X₁-Temperature (°C)	0.000179	1	0.000179	0.000136	0.0009***
X₂-pH	0.717105	1	0.717105	0.54205	0.00729**
X₃-Yeast extract (g L^-1)	0.069738	1	0.069738	0.052714	0.8215^NS
X₄-Incubation period (days)	25.37625	1	25.37625	19.18158	0.0005***
X₁X₂	0.16657	1	0.16657	0.125908	0.7277^NS
X₁X₃	0.278689	1	0.278689	0.210657	0.6528^NS
X₁X₄	0.085404	1	0.085404	0.064556	0.8029 ^NS
X₂X₃	0.081467	1	0.081467	0.06158	0.8074 ^NS
X₂X₄	0.040612	1	0.040612	0.030698	0.8633 ^NS
X₃X₄	0.075826	1	0.075826	0.057316	0.8140 ^NS
X₁²	0.395835	1	0.395835	0.299206	0.5924 ^NS
X₂²	7.268732	1	7.268732	5.494339	0.0333*
X₃²	0.080502	1	0.080502	0.06085	0.8085 ^NS
X₄²	19.98253	1	19.98253	15.10453	0.0015**
Residual	19.84424	15	1.322949
Lack of Fit	6.769842	9	0.752205	0.345196	0.9261^NS
Pure Error	13.0744	6	2.179066
Cor Total	68.40701	29

Statistically significant ***(P <0.0001), **(P < 0.01), *(P < 0.05), NS Non-significant.