Bioremoval of the synthetic dye malachite green by marine Trichoderma sp

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Table 2 Analysis of variance table (ANOVA) for response surface methodology of main effects and interacting effects of parameters in quadratic model for the dye degradation by Hypocrea lixii

Source	Sum of squares	df	Mean square	F value	p-value Prob > F
Model	42482.83	14	3034.488	20.09046	< 0.0001^***
X₁-Temperature (°C)	22.77126	1	22.77126	0.150762	0.7033^NS
X₂-pH	18.41882	1	18.41882	0.121946	0.007318^**
X₃-Yeast extract (g L^-1)	0.069738	1	0.069738	0.000462	0.9831^NS
X₄-Incubation period (days)	36055.94	1	36055.94	238.7159	< 0.0001^***
X₁X₂	0.16657	1	0.16657	0.001103	0.009739^**
X₁X₃	0.278689	1	0.278689	0.001845	0.9663^NS
X₁X₄	0.085404	1	0.085404	0.000565	0.9813^NS
X₂X₃	0.081467	1	0.081467	0.000539	0.009818^**
X₂X₄	0.040612	1	0.040612	0.000269	0.9871^NS
X₃X₄	0.075826	1	0.075826	0.000502	0.9824^NS
X₁²	298.9977	1	298.9977	1.979577	0.1798^NS
X₂²	320.0991	1	320.0991	2.119283	0.1661^NS
X₃²	118.8562	1	118.8562	0.786912	0.3890^NS
X₄²	12614.69	1	12614.69	83.51817	< 0.0001^***
Residual	2265.618	15	151.0412
Lack of Fit	1851.681	9	205.7423	2.982222	0.0984^NS
Pure Error	413.9376	6	68.9896
Cor Total	44748.45	29