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Table 1 Experimental design (CCD) of response surface methodology

From: Bioremoval of the synthetic dye malachite green by marine Trichoderma sp

Std runs (A) temperature (°C) (B) pH (C) yeast extract (g L-1) (D) incubation period (days) % of dye degradation Laccase production (UmL-1)
Experimental Predicted Experimental Predicted
1.0 10.0 3.0 5.0 0.0 0.20 0.82 0.02 0.22
2.0 40.0 3.0 5.0 0.0 1.50 2.61 0.03 0.11
3.0 10.0 11.0 5.0 0.0 1.60 2.23 0.02 0.14
4.0 40.0 11.0 5.0 0.0 1.20 4.43 0.01 0.22
5.0 10.0 3.0 9.0 0.0 0.20 0.44 0.20 0.15
6.0 40.0 3.0 9.0 0.0 1.20 2.77 0.32 0.05
7.0 10.0 11.0 9.0 0.0 1.20 2.14 0.01 0.05
8.0 40.0 11.0 9.0 0.0 3.50 4.87 0.02 0.66
9.0 10.0 3.0 5.0 15.0 87.71 93.26 2.71 2.85
10.0 40.0 3.0 5.0 15.0 87.01 94.76 2.01 2.23
11.0 10.0 11.0 5.0 15.0 87.74 94.87 2.74 2.97
12.0 40.0 11.0 5.0 15.0 86.80 96.78 1.80 2.76
13.0 10.0 3.0 9.0 15.0 87.15 92.61 2.15 2.20
14.0 40.0 3.0 9.0 15.0 86.45 94.64 1.45 2.11
15.0 10.0 11.0 9.0 15.0 86.70 94.50 1.70 2.61
16.0 40.0 11.0 9.0 15.0 87.87 96.94 2.87 2.92
17.0 5.0 7.0 7.0 7.5 88.39 78.25 3.39 2.92
18.0 55.0 7.0 7.0 7.5 88.45 72.64 3.45 2.58
19.0 25.0 1.0 7.0 7.5 86.54 76.42 1.54 1.53
20.0 25.0 15.0 7.0 7.5 87.17 72.55 2.17 1.10
21.0 25.0 7.0 3.0 7.5 88.99 78.89 3.99 3.51
22.0 25.0 7.0 11.0 7.5 88.89 78.67 3.89 3.29
23.0 25.0 7.0 7.0 7.5 87.55 86.98 2.55 3.19
24.0 25.0 7.0 7.0 22.5 87.24 66.93 2.24 1.16
25.0 25.0 7.0 7.0 7.5 88.90 86.98 3.90 3.19
26.0 25.0 7.0 7.0 7.5 88.90 86.98 3.90 3.19
27.0 25.0 7.0 7.0 7.5 88.90 86.98 3.90 3.19
28.0 25.0 7.0 7.0 7.5 88.90 86.98 3.90 3.19
29.0 25.0 7.0 7.0 7.5 88.90 86.98 3.90 3.19
30.0 25.0 7.0 7.0 7.5 86.74 86.98 1.74 3.19