Table 4 CCD experimental design
Factor 1 | Factor 2 | Factor 3 | Factor 4 | Response (Activity(IU/ml)) | ||
|---|---|---|---|---|---|---|
Run | A:CMC(g/l) | B:KH2PO4(g/l) | C:pH | D:Peptone(g/l) | Predicted | Actual |
1 | 12 | 3 | 7 | 2.5 | 2.9 | 1.86 |
2 | 10 | 2 | 6 | 1.5 | 2.1 | 1.74 |
3 | 10 | 2 | 6 | 3.5 | 1.8 | 1.95 |
4 | 12 | 3 | 3 | 2.5 | 0.8 | 0.58 |
5 | 12 | 3 | 5 | 2.5 | 5.14 | 4.97 |
6 | 12 | 3 | 5 | 2.5 | 5.08 | 4.97 |
7 | 10 | 4 | 6 | 1.5 | 0.4 | 0.21 |
8 | 10 | 2 | 4 | 1.5 | 0.6 | 1.12 |
9 | 10 | 4 | 6 | 3.5 | 0.7 | 0.5 |
10 | 10 | 2 | 4 | 3.5 | 0.2 | 0.95 |
11 | 12 | 3 | 5 | 0.5 | 2.6 | 1.96 |
12 | 12 | 3 | 5 | 2.5 | 4.84 | 4.97 |
13 | 10 | 4 | 4 | 3.5 | 1.4 | 0.85 |
14 | 12 | 3 | 5 | 2.5 | 4.78 | 4.97 |
15 | 14 | 4 | 4 | 3.5 | 0.8 | 1.47 |
16 | 8 | 3 | 5 | 2.5 | 2.6 | 2.09 |
17 | 14 | 2 | 4 | 1.5 | 0.5 | 0.89 |
18 | 12 | 5 | 5 | 2.5 | 0.2 | 0.21 |
19 | 16 | 3 | 5 | 2.5 | 3.1 | 2.38 |
20 | 12 | 3 | 5 | 2.5 | 4.8 | 4.97 |
21 | 12 | 3 | 5 | 2.5 | 5.12 | 4.97 |
22 | 14 | 2 | 4 | 3.5 | 0.1 | 0.1 |
23 | 14 | 4 | 6 | 1.5 | 2 | 1.37 |
24 | 12 | 3 | 5 | 4.5 | 2.1 | 1.51 |
25 | 12 | 1 | 5 | 2.5 | 1.8 | 0.55 |
26 | 14 | 4 | 4 | 1.5 | 2.3 | 2.18 |
27 | 14 | 2 | 6 | 3.5 | 0.7 | 1.01 |
28 | 14 | 2 | 6 | 1.5 | 0.9 | 1.43 |
29 | 10 | 4 | 4 | 1.5 | 0.9 | 0.94 |
30 | 14 | 4 | 6 | 3.5 | 1.7 | 1.03 |