Figure 1

Results of LDH assays of THP-1 cells with or without CD. Cells were treated without or with 0.15 mg/mL and 0.30 mg/mL CD for 24 h. Blank contained PBS buffer only. Supernatant from treated cells (TC) was measured by mixing 5 or 10 uL of supernatant with 595 or 590 uL of LDH reagents, respectively. Positive control (PC) consisted of lysate from control cells in the absence of CD.