Quantitative RT-PCR analysis of differentially expressed genes in Quercus suber in response to Phytophthora cinnamomi infection

Ebadzad, Ghazal; Cravador, Alfredo

doi:10.1186/2193-1801-3-613

SpringerPlus

Table 2 Description of primers used in RT-qPCR

From: Quantitative RT-PCR analysis of differentially expressed genes in Quercus suber in response to Phytophthora cinnamomi infection

Target genes	Accession number	Primer sequence	Amplicon size (bp)	Temperature (°C)	Efficiency	r²
Reverse: 5’TGAGGTTTTGAAACCCCTTG3’
Thaumatin like protein (QsTLP)^a	KF704742	Forward: 5’TCGGTCCCATGATAATAGAAG3’	207	55	90.4%	0.998
Thaumatin like protein (QsTLP)^a	KF704742	Reverse: 5’TTGCCACCGGTGCAAGTAG3’	207	55	90.4%	0.998
Chitinase (QsCHI)^a	KF704743	Forward: 5’CCGTGGTGCCTTGCCTTTG3’	177	55	96.0%	0.994
Chitinase (QsCHI)^a	KF704743	Reverse: 5’GCATCATGTGCTGAAGGCTG3’	177	55	96.0%	0.994
1,3-β-glucanase (QsGlu)^a	KF704744	Forward: 5’CACTCTCACTGGTTGTCTTG3’	175	55	93.6%	0.996
1,3-β-glucanase (QsGlu)^a	KF704744	Reverse: 5’CGGGGCTATGCAGCTGGTC3’	175	55	93.6%	0.996
Cinnamyl alcohol dehdrogenase 2 (QsCAD2)^b	AY362455	Forward: 5’AGCGGGTAGTTGTGACATCC 3’	229	55	93.8%	0.998
Cinnamyl alcohol dehdrogenase 2 (QsCAD2)^b	AY362455	Reverse: 5’AGAGGACCAATCACCATTGC3’	229	55	93.8%	0.998
Protein disulphide isomerase (QsPDI)^b	AY525090; AJ316614	Forward: 5’GCCACTGGGAAGGTTGTGAC3’	221	55	94.9%	0.998
Protein disulphide isomerase (QsPDI)^b	AY525090; AJ316614	Reverse: 5’GTTTTGCCTTCAAGCTCAGG3’	221	55	94.9%	0.998
CC-NBS-LRR resistance protein (QsRPc)^b	GU289638	Forward: 5’GTTGCAACAACCCACTTCTC3’	182	55	90.0%	0.994
CC-NBS-LRR resistance protein (QsRPc)^b	GU289638	Reverse: 5’TGAGGTTTTGAAACCCCTTG3’	182	55	90.0%	0.994
Reference genes		Primer sequence	Amplicon size (bp)	Temperature (°C)	Efficiency	r ²
RNA polymerase II (QsRPII)	KJ563261	Forward : 5’GACATAGATCCCGTTACCCA3’	168	55	92.3%	0.999
RNA polymerase II (QsRPII)	KJ563261	Reverse: 5’TTTGATTGCACCAGTAGATTC3’	168	55	92.3%	0.999
β-tubulin (QsTUB)	KJ563262	Forward: 5’GCTCACTACCCCAAGCTTT3’	187	55	90.5%	0.999
β-tubulin (QsTUB)	KJ563262	Reverse: 5’GGAACCTCTGGAGGTTAAA3’	187	55	90.5%	0.999
Eukaryotic translation initiation factor 5A (QsEIF-5A)	KJ563263	Forward: 5’GCCATGTCCGACGAGGAG3’	86	57	90.2%	1
Eukaryotic translation initiation factor 5A (QsEIF-5A)	KJ563263	Reverse: 5’CGGATGGTTCCGGCTTGC3’	86	57	90.2%	1
Clathrin adaptor complexes medium subunit family protein (QsCACs)^c	ID6728500	Forward: 5’TCTGGGAGAAGAGTGGCTACA3’	175	55	96.3%	0.993
	ID6728500	Reverse: 5’GAGCCACCATTCAAATCCT3’	175	55	96.3%	0.993

^aFrom cDNA-AFLP candidate genes; ^bfrom Coelho(2004); ^cfrom Marum et al. (2012).

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