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Table 1 Description of target and reference genes amplified in Quercus suber

From: Quantitative RT-PCR analysis of differentially expressed genes in Quercus suber in response to Phytophthora cinnamomi infection

Target genes (TDF code)

Homology

Accession number

Amplicon length (bp)

1,3-beta glucanase (QsGlu) (clone 27–2)

Ricinus communis glucan endo-1,3-β-glucosidase (XM_002518884.1)

KF704744

1230

Alcohol dehydrogenase (QsADH) (clone 29–1)

Dimocarpus longan alcohol dehydrogenase (DQ515803.1)

KF704745

1146

Thaumatin-like protein (QsTLP) (clone 59–1)

Cucumis sativus thaumatin-like protein [(LOC101227447), (XM_004163769.1)]

KF704742

750

Chitinase (QsCHI) (clone 60–1)

Fragaria vesca subsp. vesca chitinase-like protein 2-like (XM_004295058.1)

KF704743

951

Reference genes

   

RNA polymerase II (QsRPII)

-

KJ563261

954

β-tubulin (QsTUB)

-

KJ563262

1341

Eukaryotic translation initiation factor 5A (QsEIF-5A)

-

KJ563263

811

Clathrin adaptor complexes medium subunit family protein (QsCACs)

-

ID6728500

175

  1. For the target genes, amplicon sizes were calculated based on the complete coding region sequences (CDS).