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Table 4 Experimental design and results of CCD

From: Statistical optimization of fibrinolytic enzyme production by Pseudoalteromonas sp. IND11 using cow dung substrate by response surface methodology

Run

pH

Maltose

NaH2PO4

Enzyme activity (U/ml)

1

0

0

0

1028

2

0

0

0

1276

3

1

−1

−1

910

4

1.682

0

0

558

5

0

−1.681

0

683

6

0

0

0

1000

7

0

0

1.682

699

8

0

0

0

1173

9

−1

−1

−1

703

10

−1

−1

1

1186

11

1

−1

1

531

12

0

0

0

1104

13

−1

1

−1

1573

14

1

1

−1

765

15

1

1

1

462

16

0

0

−1.682

897

17

−1

1

−1

966

18

0

0

0

1055

19

−1.682

0

0

903

20

0

1.682

0

1345