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Table 2 Experimental design and results of the two level ( 2 5 ) full factorial design

From: Statistical optimization of fibrinolytic enzyme production by Pseudoalteromonas sp. IND11 using cow dung substrate by response surface methodology

Run

pH

Moisture

Maltose

Casein

NaH2PO4

Enzyme activity (U/ml)

1

1

−1

1

1

1

151

2

−1

−1

−1

−1

−1

638

3

1

1

−1

1

−1

274

4

−1

1

−1

1

1

1388

5

−1

1

−1

−1

−1

1118

6

1

−1

−1

−1

1

521

7

1

−1

−1

−1

−1

398

8

−1

1

1

−1

−1

844

9

−1

1

−1

1

−1

404

10

−1

1

−1

−1

1

1029

11

1

1

1

−1

−1

638

12

−1

−1

1

−1

1

631

13

1

1

1

1

−1

645

14

−1

−1

−1

−1

1

617

15

1

1

−1

1

−1

899

16

−1

−1

1

1

−1

576

17

1

−1

−1

1

−1

761

18

−1

−1

−1

1

1

652

19

1

−1

−1

1

−1

727

20

1

1

1

−1

1

384

21

1

−1

−1

1

1

684

22

−1

1

1

−1

1

727

23

−1

1

1

1

−1

576

24

−1

1

1

1

1

912

25

1

1

−1

−1

1

713

26

1

−1

1

−1

1

350

27

1

1

−1

1

1

363

28

−1

−1

1

1

1

1160

29

1

−1

1

1

−1

1251

30

1

1

1

1

1

89

31

−1

−1

1

−1

−1

748

32

1

−1

1

−1

−1

562