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Figure 3 | SpringerPlus

Figure 3

From: Immortalized tumor derived rat fibroblasts as feeder cells facilitate the cultivation of male embryonic stem cells from the rat strain WKY/Ztm

Figure 3

Analysis of ESC line ES21. a. ES21 cells contributed to chimeric rats after blastocyst injection and b. showed germ line transmission. c. Metaphase spreading and giemsa staining proofed that ES21 cells at passage 12 were genetically stable containing a diploid set of chromosomes. d. Teratomas with tridermal tissues (white arrow: glandular endodermal structures; black star: muscle-like mesodermal tissue; black arrow: ectodermal neural tube formation) arose from ES21 cells after subcutaneous injection into immuno-deficient mice, H&E, (2.5×).

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