Figure 2

Gender determination of pluripotency rat ES cells. a. SRY-PCR demonstrated that 8 male ESC lines out of 43 were established in two separate experiments. The rat ESC line ES21 forms undifferentiated pluripotent colonies demonstrated by b. phase contrast, c. alkaline phosphates expression, and positive stainings for d. Nanog, e. Oct4, f. SSEA-1 and g. SSEA-3 (10×); scale bar is 100 μm.