Figure 8

Comparison of Lys and Gly stimulation on the phosphorylation of Akt and AMPK, and on the activity of the autophagic-lysosomal system in C2C12 myotubes. Cells were untreated (C) or treated with 10 mM Lys (K) or 10 mM Gly (G) for 30 min. The phosphorylation levels of Ser473 in Akt (a), Thr308 in Akt (b), AMPK (c), and the ratio of LC3-II to total LC3 (LC3-I + LC3-II) (d), were determined in the lysates by Western blotting. Results are expressed as the level relative to that of the control group. Values are means with SE (n = 3–4). Different letters indicate significant differences among the groups (p < 0.05).