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Figure 4 | SpringerPlus

Figure 4

From: Lysine suppresses myofibrillar protein degradation by regulating the autophagic-lysosomal system through phosphorylation of Akt in C2C12 cells

Figure 4

Lys regulates the mTOR and AMPK pathways by activating Akt in C2C12 myotubes. Cells were treated for 30 min with DMSO (C), 10 μM Akti (Ai), 10 mM Leu (L), 10 mM Leu and 10 μM Akti (AiL), 10 mM Lys (K), or 10 mM Lys and 10 μM Akti (AiK). The phosphorylation level of 4E-BP1 (a) and AMPK (b) in the lysates was analyzed by Western blotting. The level of 4E-BP1 phosphorylation was estimated from the ratio of the γ-form to that of total 4E-BP1. Results are expressed as the level relative to the level in the control group. Representative immunoblots are shown. Values are means with SE (n = 3–4). Different letters indicate significant differences among the groups (p < 0.05).

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