Figure 3

Lys suppresses the autophagic-lysosomal system through activation of Akt in C2C12 myotubes. Cells were treated for 30 min with DMSO (C), 10 μM Akti (Ai), 10 mM Leu (L), 10 mM Leu and 10 μM Akti (AiL), 10 mM Lys (K), or 10 mM Lys and 10 μM Akti (AiK). The ratio of LC3-II to total LC3 (LC3-I + LC3-II) in the lysates was determined by Western blotting. Results are expressed as the level relative to the control group. Values are means with SE (n = 3–4). Different letters indicate significant differences among the groups (p < 0.05).