Figure 4

Influence of extracellular pH on DiSC3(5) fluorescence. Role of iron and zinc. a) Kinetics of DiSC3(5) fluorescence increase after extracellular acidification. HCl down to 6.0 was added where indicated. Curves represents 4 independent experiments. b) Influence of desferoxamine and TPEN on DiSC3(5) fluorescence evoked by pH 6.0. Con − control. DF − synaptosomes were preincubated for 30 min at 37°C with 100 μM of deferoxamine, incubation medium contains also 100 μM of deferoxamine. TPEN - synaptosomes were preincubated for 30 min at 37°C with 5 μM of TPEN, incubation medium contains also 5 μM of TPEN. Bars represent DiSC3(5) fluorescence increase within 3 minutes after additions. Data presented are mean values ± SEM of at least 4 experiments. 100% level corresponds to fluorescence increase in response to pH 6.0. **P ≤ 0.01 vs. 100%.