Figure 3

Influence of extracellular pH on JC-1 fluorescence. Role of iron and zinc. a) Kinetics of JC-1 fluorescence increase after extracellular acidification. HCl down to 6.0 was added where indicated. Curves represent 7 independent experiments. b) Influence of deferoxamine and TPEN on JC-1 fluorescence evoked by pH 6.0. Con − control. DF − synaptosomes were preincubated for 30 min at 37°C with 100 μM of deferoxamine, incubation medium contains also 100 μM of deferoxamine. TPEN - synaptosomes were preincubated for 30 min at 37°C with 5 μM of TPEN, incubation medium contains also 5 μM of TPEN. Bars represent JC-1 fluorescence increase within 4 minutes after additions. Data presented are mean values ± SEM of at least 4 experiments. 100% level corresponds to fluorescence increase in response to pH 6.0.