Figure 1

Influence of extracellular pH on DCF fluorescence in synaptosomes. Role of iron and zinc. a) Kinetics of DCF fluorescence increase after extracellular acidification. HCl down to pH 6.0 was added where indicated. Curves represent 5 independent experiments. b) Influence of deferoxamine, TPEN and ionol on DCF fluorescence evoked by pH 6.0 c) Influence of desferoxamine and TPEN on DCF fluorescence evoked by pH 7.0 d) Influence of desferoxamine, TPEN and ionol on DCF fluorescence evoked by 1 mM H₂O₂. Con − control DF − synaptosomes were preincubated for 60 min at 37°C with 100 μM of deferoxamine, incubation medium contains also 100 μM of desferoxamine. TPEN - synaptosomes were preincubated for 60 min at 37°C with 5 μM of TPEN, incubation medium contains also 5 μM of TPEN Ion - synaptosomes were preincubated for 60 min at 37°C with 200 μM of ionol, incubation medium contains also 200 μM of ionol. Bars represent DCF fluorescence increase within 4 minutes after additions. Data presented are mean values±SEM of at least 4 experiments. 100% level corresponds to fluorescence increase in response to pH 6.0 (b), pH 7.0 (c), 1 mM of H₂O₂ (d) without chelators and antioxidants (b), **P≤0.01 vs. 100%.