Figure 3

Effects of MEK or PI3K inhibitors on the production of MMP-3, VEGF and PGE2 by MH7A cells. A. MH7A cells (2 x 10⁴ cells/well) were cultured with the MEK inhibitor, PD98059 (10 - 60 nM), in the presence or absence of 100 ng/ml TNF-α. After 72 hours of incubation, MMP-3, VEGF and PGE2 production was determined by analysis of supernatants using ELISA. B. MH7A cells (2 x 10⁴ cells/well) were cultured with the PI3K inhibitor, LY294002 (10 - 60 nM), in the presence or absence of 100 ng/ml TNF-α. After 72 hours of incubation, MMP-3, VEGF and PGE2 production was determined by analysis of supernatants using ELISA. Data represent the mean ± SD of three independent experiments. *P < 0.01.