Schematic representations of the feeding and starvation regimens. Filled and open boxes indicate fed and starved conditions, respectively. Arrows directed upward indicate the sampling time . Completion of the gut purge (4-FGP and 5-FGP) was confirmed by direct observations through the integument. The prepupal state (4-FPP, 4-SPP, and 5-FPP) was diagnosed by apolysis of the integument and the refractoriness of insects when stimulated with tweezers. Pupation occurred approximately 10 days after the gut purge (4-FGP and 5-FGP) and 5 days after becoming prepupa (4-FPP, 4-SPP, and 5-FPP). Numerals in parentheses indicate days after ecdysis.