Figure 4

GCs showed different effects on proliferation of the MAP2-positive neurons under H ₂ O ₂ -treated conditions. (a) Representative pictures of NPCs were stained with an antibody against MAP2 (red) and nuclear counterstain DAPI (blue). The cells were pre-exposed to H₂O₂ and treated with 50 μM of the indicated GCs. Phase, phase contrast image. Scale bar, 100 μm. (b) Quantification of MAP2 positive neurons using ImageJ. P values were calculated by comparing GC treated samples with the samples treated with H₂O₂ alone (n = 3). *P < 0.05, **P < 0.01. (One-way ANOVA with Tukey-Kramer). DEX: dexamethasone, BET: betamethasone, HDC: hydrocortisone. Note that HDC alone promoted significant cell proliferation of MAP2 positive neurons under oxidative stress.