Table 1 Primers used in this study
From: Creatine metabolism differs between mammals and rainbow trout (Oncorhynchus mykiss)
Primer name | Source sequence | Sequence (5′–3′) |
|---|---|---|
Amplification of coding sequences from rainbow trout | ||
OM_GATM_CDS_f | BX868137 | CCGCCGCTAGAATATCCCAAAT |
OM_GATM_CDS_r | DV201821 | TGCAGATTGTTGATTGGGACTTT |
OM_GAMT_CDS_f | CR374244 | AGACAGCAACTCCGTCCATC |
OM_GAMT_CDS_r | BX076691 | GCACTTGAGAAGGCATGACA |
OM_CKM_CDS_f | CT569995 | GGCTCTGGTGAACAGGATCTGA |
OM_CKM_CDS_r | CT569258 | GGTTGGCTCAATGGCACATAAC |
Amplification of SLC6A8-fragment from rainbow trout a | ||
OM_SLC6A8_frag_f | cf. methods | CCTCCATGGTGATTGTSTTCT |
OM_SLC6A8_frag_r | cf. methods | CRCTGGCWGAGTAGTAGTCAAA |
Quantification of transcripts | ||
OM_GATM_qPCR_f | HG315738 | ACCTCTACTGGCATGTATGCTG |
OM_GATM_qPCR_r | HG315738 | CTTGGCACCCTTTCTGAAGTAC |
OM_GAMT_qPCR_f | HG315739 | TCGACAACATGTTCCAGGAGAC |
OM_GAMT_qPCR_r | HG315739 | GCAGTGGCATCAAGCCATTTCA |
OM_CKB_qPCR_f | FJ548753 | ATAACCCAGGCCACCCCTTCA |
OM_CKB_qPCR_r | FJ548753 | TGGGTTCAGGTCGGTCTTGTG |
OM_CKM_qPCR_f | HG315740 | TGCGTTGGTCTGAAAAGGATTGA |
OM_CKM_qPCR_r | HG315740 | TCTCCTCAAACTTGGGGTGTGT |
OM_SLC6A8_qPCR_f | HG315741 | GGAAGCCCAGGTGTGGATTGA |
OM_SLC6A8_qPCR_r | HG315741 | AAAGAAACTGGTCCCACTGTTGA |
OM_EEf1A1_qPCR_f | NM_001124339 | TGATCTACAAGTGCGGAGGCA |
OM_EEf1A1_qPCR_r | NM_001124339 | CAGCACCCAGGCATACTTGAA |
OM_RPS5_qPCR_f | NM_001160519 | ATGACATCTCACTGCAGGATTAC |
OM_RPS5_qPCR_r | NM_001160519 | ATCAGCTTCTTGCCGTTGTTGC |