Figure 3
From: Creatine metabolism differs between mammals and rainbow trout (Oncorhynchus mykiss)
Representative gels of semiquantitative PCR of five transcript fragments encoding factors of the creatine pathway. Expression of GATM, GAMT, SLC6A8, CKB and CKM in kidney, liver, brain and muscle was studied using semiquantitative PCR. EEF1A1 was used as a reference gene. The gel photos show results of one import trout. The cycle number was higher for GATM and SLC6A8 (35 cycles) than for the other genes (30 cycles). The lower bands represent EEF1A1 and the upper bands the target genes.