Figure 4

TTP interacts with BDNF mRNA and regulates endogenous BDNF levels in myogenic C2C12 cells. a. Outline of an experiment with C2C12 cells. Left: illustrative image of undifferentiated cells (myoblasts) at day 1; Right: illustrative image of differentiated cells (myotubes) at day 5. GM-growth medium, DM-differentiation medium. b. TTP but not BRF1 and BRF2 mRNA levels are upregulated in differentiating myotubes at day 5. **p < 0.01, N = 2-4, with 2–3 repeats per experiment. c. Endogenous BDNF mRNA co-immunoprecipitates with endogenous TTP protein in differentiating C2C12 cells, N = 2 experiments. **p = 0.01. d. Reduction in TTP mRNA levels after siRNA-mediated TTP knock-down at day 5, N = 4 experiments with 2–3 repeats per experiment, ** p < 0.01. e. Increase in BDNF protein levels after siRNA-mediated TTP knock-down (c) in the cell-culture medium at day 5. N = 3 experiments with two repeats per experiment, ** p < 0.01. Error bars indicate SD on all graphs.