Figure 2

Effects of ARE-BPs on expression from luciferase reporter containing BDNF-L or BDNF-S. a. Effects of TTP, BRF1, BRF2, ELAVL1, ELAVL2 and AUF1 on the expression of luciferase reporter containing BDNF-L (Luc-BDNF-L) compared to Green Fluorescent Protein (GFP) and empty vector (pDEST40) as negative controls in HEK-293 cells, N = 2-8 experiments with 3–4 repeats per experiment, ***p < 0.001, #p < 0.035, Error bars indicate SD. b. Comparison of effects of TTP, BRF1 and BRF2 on Luc-BDNF-L and Luc-BDNF-S expression in HEK-293 cells revealed no statistically significant difference between BDNF-L and BDNF-S, N = 3-4, experiments with 4 repeats per experiment, ***p < 0.001. Error bars indicate SD. c. Effect of TTP expression on Luc-BDNF-S mRNA turn over in the HEK-293 cells upon Actinomycin D (1 μg/ml) treatment at the indicated time points, N = 2 experiments, a representative experiment is shown. BDNF-S and β-actin mRNA was detected with semi-quantitative PCR.