Table 1 Primer sequences of PCR-CTPP for VKORC1 G-1639A and CYP2C9 A1075C
Primers | Sequences | Tm (°C) |
|---|---|---|
VKORC1 G-1639A F1 | 5′ CAC AGA CGC CAG AGG AAG AGA G | 64.0 |
VKORC1 G-1639A R1 | 5′ CGT GAG CCA CCG CAC CT | 65.1 |
VKORC1 G-1639A F2 | 5′ GAA GAC CTG AAA AAC AAC CAT TGG CCG | 64.4 |
VKORC1 G-1639A R2 | 5′ CTC AGC CTC CCA AGT AGT TTG G | 62.1 |
CYP2C9 A1075C F1 | 5′ CCA GGA AGA GAT TGA ACG TGT GAT TG | 61.6 |
CYP2C9 A1075C R1 | 5′ TGG TGG GGA GAA GGT CAA T | 61.3 |
CYP2C9 A1075C F2 | 5′ GCA CGA GGT CCA GAG ATA CC | 61.7 |
CYP2C9 A1075C R2 | 5′ GAG TTA TGC ACT TCT CTC ACC CG | 61.1 |