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Table 1 Primer sequences of PCR-CTPP for VKORC1 G-1639A and CYP2C9 A1075C

From: A PCR method for VKORC1 G-1639A and CYP2C9 A1075C genotyping useful to warfarin therapy among Japanese

Primers Sequences Tm (°C)
VKORC1 G-1639A F1 5′ CAC AGA CGC CAG AGG AAG AGA G 64.0
VKORC1 G-1639A R1 5′ CGT GAG CCA CCG CAC CT 65.1
VKORC1 G-1639A F2 5′ GAA GAC CTG AAA AAC AAC CAT TGG CCG 64.4
VKORC1 G-1639A R2 5′ CTC AGC CTC CCA AGT AGT TTG G 62.1
CYP2C9 A1075C F1 5′ CCA GGA AGA GAT TGA ACG TGT GAT TG 61.6
CYP2C9 A1075C R1 5′ TGG TGG GGA GAA GGT CAA T 61.3
CYP2C9 A1075C F2 5′ GCA CGA GGT CCA GAG ATA CC 61.7
CYP2C9 A1075C R2 5′ GAG TTA TGC ACT TCT CTC ACC CG 61.1
  1. CYP = Cytochrome P450, PCR-CTPP = Polymerase chain reaction with confronting two-pair primers, Tm = Melting temperature, VKORC1 = Vitamin K epoxide reductase complex, subunit 1.
  2. Polymorphic bases are indicated in bold type.