Figure 1

Logic of PCR-CTPP. a, size for X allele; b, size for Y allele; c = a + b - (d - 1), size between primers 1Â F and 2R; d, Sum of the size of primers 2 F and 1R. PCR-CTPP = Polymerase chain reaction with confronting two-pair primers.
Logic of PCR-CTPP. a, size for X allele; b, size for Y allele; c = a + b - (d - 1), size between primers 1Â F and 2R; d, Sum of the size of primers 2 F and 1R. PCR-CTPP = Polymerase chain reaction with confronting two-pair primers.