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Figure 7 | SpringerPlus

Figure 7

From: The ER stress inducer DMC enhances TRAIL-induced apoptosis in glioblastoma

Figure 7

Sensitivity of A172 to TRAIL-induced apoptosis is slightly enhanced by down-regulation of survivin. (A) Western blot analysis of survivin siRNA transfected A172 cells treated with or without 10 ng/ml rhTRAIL WT or rhTRAIL D269H/E195R for 5h. Lysates were tested for survivin down-regulation and apoptosis induction by PARP cleavage. β-actin served as a loading control. (B) A172 cells transfected with survivin siRNA were treated with 0–100 ng/ml rhTRAIL WT or rhTRAIL D269H/E195R. Cell viability was assessed after 24h using MTS assay. The graphs are corrected for control or survivin knockdown induced cytotoxicity. Presented data are representative for at least three independent experiments and mean cell viability levels ± S.E.M. are shown.

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