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Figure 6 | SpringerPlus

Figure 6

From: The ER stress inducer DMC enhances TRAIL-induced apoptosis in glioblastoma

Figure 6

DMC mainly down-regulates the anti-apoptotic protein survivin. (A) Flow cytometry analysis was performed after 24h of 0, 25 or 50 μM DMC treatment to measure the cell surface expression of TRAIL receptors in A172 and U87 cells, expressed as the Mean Fluorescence Intensity (MFI) ratio. Error bars represent S.E.M. of three independent experiments. (B) Western blot analysis of A172 (5h) and U87 (24h) cells treated with either rhTRAIL WT (0, 10 or 100 ng/ml) and/or DMC (0, 25 or 50 μM). C-Flip, Bcl-2 and survivin protein expression levels were tested. β-actin served as a loading control. Presented data are representative for at least two independent experiments.

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