Process evaluation of electron beam irradiation-based biodegradation relevant to lignocellulose bioconversion

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Table 1 Downstream evaluation for scale-up in advanced EBIBB program

Type	Inhibitory byproducts			Hydrolysis index^d(per 100 g biomass)			Fermentation index^e(per 100 g biomass)
	HMF^c(w/w, %)	Furfural^c(w/w, %)	Acetate(g/L)	EBIBB for 5 days	EBIBB for 10 days	EBIBB for 15 days	EBIBB after 10 days
EBIBB	Not detected	Not detected	< 0.05	≤ 55.2% (17.3 ± 0.2 g glucose)	≤ 65.5% (20.7 ± 0.1 g glucose)	≤ 61.2% (19.1 ± 0.2 g glucose)	≤ 62.9% (10.0 ± 0.2 g ethanol) at 10 day
NC^a	Not detected	Not detected	< 0.02	≤ 48.3% (15.4 ± 0.2 g glucose)	≤ 58.4% (18.7 ± 0.2 g glucose)	≤ 64.8% (20.8 ± 0.2 g glucose)	≤ 62.5% (9.9 ± 0.2 g ethanol) at 15 day
Untreated^b	Not detected	Not detected	< 0.04	≤ 27.1%^b (8.7 ± 0.1 g glucose)			≤ 29.5%^b (4.7 ± 0.1 g ethanol)

^anegative control; biodegradation without the irradiation.
^bwithout either the EBI or the biodegradation.
^cdetermined as $\frac{g of HMF of treated RS}{g of initial weight of RS} \times 100$ and $\frac{g furfural of treated RS}{g of initial weight of RS} \times 100$
^dthe yield of theoretical maximum glucose based on the enzymatic digestibility after 96 h.
^ethe yield of theoretical maximum ethanol based on the simultaneous saccharification and fermentation after 72 h.