Figure 4From: RNA interference-based gene silencing of phytoene synthase impairs growth, carotenoids, and plastid phenotype in Oncidium hybrid orchidCarotenoid biosynthesis pathway in Oncidium orchid and analysis of the expression level of OgPSY in transgenic Oncidium . (A) The scheme of carotenoid biosynthesis pathway in Oncidium orchid. *Indicates the target gene of PSY for RNA interference-based gene silencing. (B) Relative transcript level of OgPSY gene in wild type and OgPSY RNAi transgenic Oncidium orchid. GGDS: GGPP synthase; PSY: phytoene synthase; PDS: phytoene desaturase; ZDS: zeaxanthin desaturase; LCY: lycopene cyclase. ZEP: zeaxanthin epoxidase. Gene transcript levels were measured by RT-qPCR. Transcript levels were calculated and normalized with respect to ubiquitin mRNA. The experiment was repeated three times.Back to article page