Metabolism under hypoxia in Tm1 murine melanoma cells is affected by the presence of galectin-3, a metabolomics approach

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Table 2 Summary of results obtained from ANOVA and Fisher’s test

*Metabolite*	*l.s.d.*	\|Nhh – Ghh\|	\|Nhh – Ghc\|	\|Ghh – Ghc\|
Lactate	0.03048	0.04277	0.06494	0.02217
Free choline	0.00677	0.00345	0.00945	0.00600
PC/GPC	0.01748	0.00876	0.02713	0.01838
Citrate (P14)	0.01680	0.01035	0.01916	0.02950
P27	0.07465	0.11239	0.01375	0.12614
Creatine	0.01392	0.01980	0.03537	0.05517
Glutamate	0.00257	0.00300	0.00616	0.00916
Succinate	0.00077	0.00003	0.00198	0.00200
Taurine	0.00786	0.01243	0.01271	0.02514
Glycine	0.00324	0.00466	0.00605	0.01071
AMP, ADP, ATP, GDP, NADP⁺	0.01087	0.01098	0.01139	0.02237
Alanine	0.00461	0.00075	0.01343	0.01418

Parameters used were: α = 5% (significance level), N = 24 (total of elements in multivariate matrix), k = 8 (groups), n = 3 (elements by group) and Fisher’s coefficient of 2.12. Least significant difference (l.s.d.), |Nhh – Ghh|, |Nhh – Ghc| and |Ghh – Ghc| were calculated. These differences were considered statistically significant only when they were greater than l.s.d.