Table 2 Optimization of CTAB DNA extraction buffer components for Passiflora foetida
Sr no | Parameters | Tested range | Optimum conditions | Inferences |
|---|---|---|---|---|
1 | CTAB | W, 1%, 2%, 3%, 4%, 5% | 2%, | Disrupts cell membranes |
2 | PVP | W, 1%, 2%, 3%,3.5%, 4%, 4.5%, 5% | 1%, | Effects DNA quality |
3 | Water bath temperature | 30°C, 50°C, 60°C, 65°C, 70°C | 70°C | Less temperature increase contamination chances |
4 | Duration on water bath | Half hrs, 1 hrs | 1 hrs | Less duration effects DNA purity |