Table 1 Primers used in molecular confirmation study

VMP 23-1-F

5′ CTTTTGGGTCATCCTRGA 3′

48°C

1,051 bp

Identification (Bauernfeind et al., 1998)

MP 23-2-R

5′ TCCTACCATGCGAGACT 3′

BPTTS1-F

5′CGTCTCTATACTGTCGAGCAATCG 3′

58°C

548 bp

Identification (Novak et al., 2006)

BPTTS1-R

5′ CGTGCACACCGGTCAGTATC 3′

F8

5′ AGTTTGATCCTGGCTCAG 3′

50°C

1,488 bp

16S sequencing (Gee et al., 2003)

R1492

5′ ACCTTGTTACGACTT 3′

fli C-F

5′ CTCGGATCCAACAGCAAC 3′

52°C

1,167 bp

PCR-RFLP (Primer designed)

fli C-R

R- 5′ TATTGCAGGTACCTTCAG 3′