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Table 1 hPGHSs subjected to N -glycosylation analysis

From: N-glycosylation site occupancy in human prostaglandin H synthases expressed in Pichia pastoris

Recombinant vector

Native/ optimised sequence

Signal sequence

Activity1

optC-termHis6PGHS-1 pHIL-D2

Optimised

Native

0

N-termPGHS-2/optC-termHis6 PGHS-1 pHIL-D2

Optimised, except the sequence of PGHS-2

PGHS-2

215 ± 19

C-termHis6PGHS-2-sp pHIL-S1

Native

Yeast

577 ± 21

  1. 1pmoles of prostaglandins formed in 10 min per 1 mg (wet weight) of yeast cells. Mean ± S.D. (n = 5).