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Figure 2 | SpringerPlus

Figure 2

From: N-glycosylation site occupancy in human prostaglandin H synthases expressed in Pichia pastoris

Figure 2

Example fragmentation spectra of a PGHS-1‚ÄČN -glycosylation site containing peptide TGFYGENCSTPEFLTR, indicating variable glycosylation. High mass accuracy of precursor peptides combined with MS/MS fragment ions readily captures expected 16O/18O dependent mass-shifts and confidently identifies that the site has variable glycosylation state in the recombinant protein. y-series fragment ions which pinpoint the modification within the sequence are indicated with red arrows (note, that beyond y9-fragment the masses are shifted relative to the unmodified fragments according to the incorporated oxygen atom).

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