Table 1 The yield of recombinant β-mannanase in different cell compartments of E. coli (from 100 ml culture)
Cell compartments | Activity | Protein concentration | Specific activity |
|---|---|---|---|
(U per ml) | (mg per ml) | (u per mg) | |
Periplasmic space | 497.62 | 0.03 | 16,587.33 |
Cell lysate | 45.14 | 0.05 | 902.50 |